Pulsed Electric Fields for Valorization of Platelets with No Therapeutic Value towards a High Biomedical Potential Product—A Proof of Concept

Nowadays, the standard media used in clinical-scale mesenchymal stem cell (MSC) production to supply hundreds of clinical trials uses animal-derived components as supplements, which raises several health concerns. Consequently, development xeno-free supplements has emerged. In current study, effect pulse electric field (PEF) application platelet concentrates (PC) with no therapeutic value for producing releasates (PR) able sustain ability bone marrow-MSCs (BM-MSCs) self-renew and differentiate was tested. It demonstrated that PEF PC induces activation growth factor (GF) release, namely PDGF, FGF, IGF, TGF-β. The highest GF release observed TGF-β, achieving similar levels those attained lysates (PL). BM-MSCs expanded presence PR obtained by (7 pulses 10 12.5 kV/cm) (PR PEF) retained characteristic MSC cell-surface markers, proliferate into osteogenic, adipogenic, chondrogenic lineages. this evidence is provided represents a suitable alternative fetal bovine serum (FBS) use production.